Alanine-scanning mutagenesis of proline residues in NS5A domain II.

aReplication capacity of the WT (fold increase from 4 h to day 3) was set at 100%, and the following scales were used: –, 0–10%; +, 11–50%; ++, 51–75%; +++, 76–100%; ++++, >100%.
bThe ratio of replication capacity in sh-A161 cells to that in sh-Luc cells was calculated and presented for all the D316E/Y317N mutation–based genomes. Standard errors of two independent experiments are shown.