Quantitative MSI evaluation of pathogen inactivation strategies for spatial lipidomics - positive mode

In this study, we evaluated the impact of gamma irradiation, heat inactivation and neutral buffered formalin fixation on phospholipid profiles in mouse kidney tissue using MALDI-MSI. By combining class-level normalization to deuterated standards, relative percentage change analysis, and receiver operating characteristic (ROC)–based species discrimination, we provide a quantitative framework to assess the robustness and sensitivity of lipid measurements across commonly used tissue inactivation methods. This dataset is the positive ion mode SCiLS assembly exported in the imZML format. The negative ion mode data is located here: 10.5281/zenodo.21481616 (linked below). Please write to the authors to request raw or supporting data due to data volume. Reasonable requests will be granted.