Effects of LL-37 derived peptides on cell viability as assessed by LDH assay.

a MDCK or HBTE were treated 12μM of the indicated peptides along with virus (Phil82 strain) or without virus. The experiments were carried out in the identical manner as the infectious focus or qPCR assays. The percent of viable cells was measured using an LDH assay as described in methods. Where indicated by a * samples had significantly reduced viability compared to control. Results are mean±SEM of 3 or 4 experiments, except in the case of LL-23V9 and HBTE cells where n = 1.Effects of LL-37 derived peptides on cell viability as assessed by LDH assay.以12μM的指定多肽处理MDCK细胞（Madin-Darby Canine Kidney cells）或HBTE细胞（人支气管气管上皮细胞），同时添加或不添加Phil82毒株病毒。本实验的操作流程与感染灶测定法（infectious focus assay）或qPCR实验完全一致。按照方法部分所述的乳酸脱氢酶检测法（LDH assay），对活细胞百分比进行测定。带有*标记的样本，其细胞活力与对照组相比显著降低。实验结果以均值±标准误（SEM）表示，除LL-23V9多肽与HBTE细胞组的实验重复数n=1外，其余均为3或4次独立实验的结果。本部分为通过LDH检测法评估的LL-37衍生多肽对细胞活力的影响。