File S1 - Role of Nicotine Dependence in the Association between the Dopamine Receptor Gene DRD3 and Major Depressive Disorder

Figure S1 and Tables S1–S4. Figure S1. A) DRD3 gene structure, B) genotyped SNPs, C) D' in the HapMap CEPH data (NCBI Build 36), D) r2 in the HapMap CEPH data, E) D' in the study sample (non-related individuals; one per family), F) r2 in the study sample. Table S1. Correlations between the included phenotypes. Correlations were computed by polychoric (tetrachoric and point biserial) and spearman correlation. Number of individuals varies from 1326 to 1428 depending on presence of missing values. Table S2. Marker quality controls. Table S3. Association analysis results (p-values) for all dopamine receptor genes. The study-specific P-value threshold for significant and suggestive association is 0.00042 and 0.0014, respectively. Table S4. a. The association of rs2399496, rs3732790 and rs2134655 with nicotine dependence (ND) and Major Depressive Disorder (MDD) in the Australian NAG-OZALC sample. Age, sex, and principal components (for population stratification) were used as covariates. All results are based on recessive models. b. The associations of rs2399496, rs3732790 and rs2134655 with nicotine dependence (ND) and Major Depressive Disorder (MDD) in the NTR-NESDA sample. Age, sex, and principal components (for population stratification) were used as covariates. All results are based on recessive models. c. The associations of rs2399496, rs3732790 and rs2134655 with nicotine dependence (ND) and Major Depressive Disorder (MDD) in the FT12 sample. Age and sex were used as covariates. All results are based on recessive models. d. The associations of rs2399496, rs3732790 and rs2134655 with nicotine dependence (ND1) and Major Depressive Disorder (MDD) in the T2000 sample. Age and sex were used as covariates. All results are based on recessive models. (DOCX)