File S1 - Role of Nicotine Dependence in the Association between the Dopamine Receptor Gene DRD3 and Major Depressive Disorder

Figure S1 and Tables S1–S4. Figure S1. A) DRD3 gene structure, B) genotyped SNPs, C) D' in the HapMap CEPH data (NCBI Build 36), D) r2 in the HapMap CEPH data, E) D' in the study sample (non-related individuals; one per family), F) r2 in the study sample. Table S1. Correlations between the included phenotypes. Correlations were computed by polychoric (tetrachoric and point biserial) and spearman correlation. Number of individuals varies from 1326 to 1428 depending on presence of missing values. Table S2. Marker quality controls. Table S3. Association analysis results (p-values) for all dopamine receptor genes. The study-specific P-value threshold for significant and suggestive association is 0.00042 and 0.0014, respectively. Table S4. a. The association of rs2399496, rs3732790 and rs2134655 with nicotine dependence (ND) and Major Depressive Disorder (MDD) in the Australian NAG-OZALC sample. Age, sex, and principal components (for population stratification) were used as covariates. All results are based on recessive models. b. The associations of rs2399496, rs3732790 and rs2134655 with nicotine dependence (ND) and Major Depressive Disorder (MDD) in the NTR-NESDA sample. Age, sex, and principal components (for population stratification) were used as covariates. All results are based on recessive models. c. The associations of rs2399496, rs3732790 and rs2134655 with nicotine dependence (ND) and Major Depressive Disorder (MDD) in the FT12 sample. Age and sex were used as covariates. All results are based on recessive models. d. The associations of rs2399496, rs3732790 and rs2134655 with nicotine dependence (ND1) and Major Depressive Disorder (MDD) in the T2000 sample. Age and sex were used as covariates. All results are based on recessive models. (DOCX)补充图S1及补充表S1至S4。 补充图S1：A) DRD3基因结构，B) 经基因分型的单核苷酸多态性（SNPs），C) HapMap CEPH数据集（NCBI Build 36版本）中的连锁不平衡参数D'值，D) HapMap CEPH数据集中的连锁不平衡参数r²值，E) 本研究样本（非相关个体；每个家系选取1例）中的D'值，F) 本研究样本中的r²值。 补充表S1：纳入表型间的相关性。相关性通过多相关（四分相关及点二列相关）与斯皮尔曼相关计算得到。由于存在缺失值，有效个体数量在1326至1428之间不等。 补充表S2：标记质量控制情况。 补充表S3：所有多巴胺受体基因的关联分析结果（p值）。本研究设定的显著性关联与提示性关联的P值阈值分别为0.00042与0.0014。 补充表S4： a. 澳大利亚NAG-OZALC样本中rs2399496、rs3732790及rs2134655与尼古丁依赖（ND, Nicotine Dependence）及重性抑郁障碍（MDD, Major Depressive Disorder）的关联分析。以年龄、性别及用于校正群体分层的主成分作为协变量，所有结果均基于隐性遗传模型。 b. NTR-NESDA样本中rs2399496、rs3732790及rs2134655与尼古丁依赖（ND, Nicotine Dependence）及重性抑郁障碍（MDD, Major Depressive Disorder）的关联分析。以年龄、性别及用于校正群体分层的主成分作为协变量，所有结果均基于隐性遗传模型。 c. FT12样本中rs2399496、rs3732790及rs2134655与尼古丁依赖（ND, Nicotine Dependence）及重性抑郁障碍（MDD, Major Depressive Disorder）的关联分析。以年龄与性别作为协变量，所有结果均基于隐性遗传模型。 d. T2000样本中rs2399496、rs3732790及rs2134655与尼古丁依赖（ND1, Nicotine Dependence 1）及重性抑郁障碍（MDD, Major Depressive Disorder）的关联分析。以年龄与性别作为协变量，所有结果均基于隐性遗传模型。(DOCX)