Complete DNA Sequence of Kuraishia capsulata Illustrates Novel Genomic Features among Budding Yeasts (Saccharomycotina). AQF

We sequenced, assembled and annotated the nuclear genome of the haploid type strain of Kuraishia capsulata CBS1993T, a nitrate assimilating Saccharomycetales of uncertain taxonomy, isolated from tunnels of insect larvae underneath coniferous barks and characterized by its copious production of extracellular polysaccharides. The sequence is composed of 7 scaffolds, one per chromosome, totaling 11.4 Mb and containing 6,029 protein-coding genes, ca. 13.5 % of which being interrupted by introns. This yeast appears phylogenetically related with the few other nitrate assimilating yeasts sequenced so far, Ogataea angusta, O. parapolymorpha and Dekkera bruxellensis with which it shares a very reduced number of tRNA genes, a novel tRNA sparing pattern, and a common nitrate assimilation cluster, three specific features to this group of yeasts. The complete absence of conservation of synteny between K. capsulata and any other yeast genome described so far, including the three other nitrate-assimilating species, validates the interest of this species for long range evolutionary genomic studies among Saccharomycotina yeasts