Single-cell RNA-seq profiles of lung adenocarcinoma patients and tumor-bearing mice

single-cell RNA sequencing (scRNA-seq) profiles from eight patients with lung adenocarcinoma (LUAD) and four samples of tumor tissues from tumor bearing mice were performed. By integrating other scRNA-seq data and clinical information, we identified activated adaptive immune responses in older patients, reflected by enriched dysfunctional T cell signature scores and immune checkpoint molecules. Our study shows increased efficacy of immune checkpoint blockade therapy in older patients, addressing the prominent role of age when considering immunotherapy.

本研究完成了8例肺腺癌（lung adenocarcinoma, LUAD）患者与4例荷瘤小鼠肿瘤组织样本的单细胞RNA测序（single-cell RNA sequencing, scRNA-seq），获得了相应的测序表达谱数据。通过整合其他单细胞RNA测序数据与临床信息，本研究发现老年患者体内存在激活的适应性免疫应答，该应答可通过功能失调性T细胞特征评分升高以及免疫检查点分子富集得到反映。本研究证实，老年患者的免疫检查点阻断治疗疗效显著提升，明确了年龄在免疫治疗考量中的重要作用。