Comparative RNA-seq Analysis of Diabetic Wound Healing Treated with Adipose-Derived Stem Cell-Derived Extracellular Vesicles Versus PBS

This project aims to investigate the therapeutic potential of adipose-derived stem cell extracellular vesicles (ADSC-EVs) in the treatment of diabetic wounds using a diabetic rat model. Diabetes mellitus is often associated with impaired wound healing, which poses significant challenges in clinical management. Extracellular vesicles (EVs) derived from adipose tissue stem cells have emerged as promising therapeutic agents due to their ability to deliver bioactive molecules such as RNA and proteins to recipient cells.In this study, diabetic wound tissues treated with ADSC-EVs and phosphate-buffered saline (PBS; control group) were collected for RNA sequencing analysis. The primary goal of the study was to examine differential gene expression patterns and identify molecular pathways potentially involved in enhanced wound healing processes mediated by ADSC-EVs.RNA-seq technology was employed to uncover transcriptional responses of diabetic wound tissues following ADSC-EV treatment. The analysis not only reveals key differences between the ADSC-EV and PBS treatment groups but also sheds light on potential regulatory mechanisms and therapeutic targets in diabetic wound repair. The study contributes to a better understanding of the biological impact of ADSC-EVs and their role in promoting tissue regeneration in diabetic conditions.