The sequences of forward and backward primers, restriction enzymes for genotyping of the ABCG5/G8 SNPs.

X, mismatching nucleotides (T modified C) in forward primer, Xho I could find the restriction site (5′-C!TCGAG-3′) in the amplicon sequence. Y, mismatching nucleotides (T modified G) in forward primer, Ban I could find the restriction site (5′-G!GYRCC-3′) in the amplicon sequence. Z, mismatching nucleotides (CT modified GC) in backward primer, Bgl I could find the restriction site (5′-GCCNNNN!NGGC-3′) in the amplicon sequence.