The sequences of forward and backward primers, restriction enzymes for genotyping of the ABCG5/G8 SNPs.

X, mismatching nucleotides (T modified C) in forward primer, Xho I could find the restriction site (5′-C!TCGAG-3′) in the amplicon sequence. Y, mismatching nucleotides (T modified G) in forward primer, Ban I could find the restriction site (5′-G!GYRCC-3′) in the amplicon sequence. Z, mismatching nucleotides (CT modified GC) in backward primer, Bgl I could find the restriction site (5′-GCCNNNN!NGGC-3′) in the amplicon sequence.X代表正向引物中存在错配核苷酸（将T替换为C）的情形，此时扩增子（amplicon）序列中可被限制性内切酶Xho I识别的限制性酶切位点为5′-C!TCGAG-3′。 Y代表正向引物中存在错配核苷酸（将T替换为G）的情形，此时扩增子序列中可被限制性内切酶Ban I识别的限制性酶切位点为5′-G!GYRCC-3′。 Z代表反向引物中存在错配核苷酸（将CT替换为GC）的情形，此时扩增子序列中可被限制性内切酶Bgl I识别的限制性酶切位点为5′-GCCNNNN!NGGC-3′。