Effects of etravirine on HIV-1 reverse transcriptase, recovered from plasma of HIV-1 infected patients.

*Two recombinant reverse transcriptase, (BH10)-wild-type (WT) and its mutant form L100I, were used as references. Based on the IC50 value of BH10-wild-type (1.0±0.18 µM), which was included in every run, the IC50 value and the fold-difference in susceptibility of each isolate were determined. A fold-difference of Abbreviations: NA, not applicable; IC50, inhibitory effect; ETR etravirine.**Genotype result was obtained six months earlier. The results of three genotypic scoring systems (Stanford, Monogram Weighted Score, Tibotec Weighted Genotype Score) predicting ETR susceptibility are presented. s: sensitive; i: intermediate; r: resistant. A Stanford score of 0-* 本研究采用两种重组逆转录酶（reverse transcriptase）——(BH10)野生型（WT）及其突变体L100I作为参照样本。基于每轮实验均纳入的BH10野生型的半数抑制浓度（IC50）值（1.0±0.18 µM），我们测定了每株分离株的IC50值及其敏感性倍数差异。敏感性倍数差异相关缩写说明：NA（not applicable，不适用）；IC50（inhibitory effect，抑制效应）；ETR即依曲韦林（etravirine）。** 基因型检测结果提前六个月获得。本研究呈现了三种基因型评分系统（斯坦福评分系统、Monogram加权评分、Tibotec加权基因型评分）预测依曲韦林敏感性的结果。其中s代表敏感（sensitive），i代表中介（intermediate），r代表耐药（resistant）。斯坦福评分0至