LPAR5 restrains sepsis-induced immunosuppression through STING-mediated dendritic cell ferroptosis and ISG+ cDC2 subset

This dataset contains processed single-cell RNA-seq count matrices generated for the study “LPAR5 restrains sepsis-induced immunosuppression through STING-mediated dendritic cell ferroptosis and an ISG⁺ cDC2 subset”. Spleen cells from wild-type sham, wild-type CLP, and DC-Lpar5−/− CLP mice were profiled using 10x Genomics single-cell 3′ RNA-seq. Gene-by-cell UMI count matrices after Cell Ranger processing, EmptyDrops cell calling, and quality control are provided for each library. These matrices were used for all downstream analyses reported in the manuscript (clustering, cell type annotation, differential expression, CellChat, AUCell, trajectory analysis, etc.). Raw FASTQ files are not available from the sequencing facility; therefore, only processed count matrices are archived here to enable independent re-analysis.本数据集包含为研究"LPAR5通过干扰素基因刺激因子（STING）介导的树突状细胞铁死亡及干扰素刺激基因阳性经典树突状细胞2亚型（ISG⁺ cDC2）亚群抑制脓毒症诱导的免疫抑制"所生成的经处理的单细胞RNA测序计数矩阵。野生型假手术组、野生型盲肠结扎穿刺（CLP）组及DC-Lpar5基因敲除CLP组小鼠的脾脏细胞，通过10x Genomics单细胞3'端RNA测序完成分型分析。针对每个文库，均提供了经Cell Ranger处理、EmptyDrops细胞检出及质量控制后的基因-细胞唯一分子标识符（UMI）计数矩阵。这些矩阵被用于本论文中报道的全部下游分析，包括聚类分析、细胞类型注释、差异表达分析、CellChat、AUCell、轨迹分析等。测序机构未提供原始FASTQ格式文件，因此本存档仅提供处理后的计数矩阵，以供独立重分析使用。