Raw Diffraction Images Of Eukaryotic Mate Transporter (Atdtx14)

Multidrug And Toxic compound Extrusion (MATE) transporter exports xenobiotics by using the gradient of H⁺. The crystals were obtained within the lipidic cubic phase. 288+85 (Auto+manual) small-wedge (5-20°/crystal) datasets collected from loop-harvested microcrystals using MX225HS CCD detector at a wavelength of 1 Å on BL32XU, SPring-8. The crystals belonged to space group P2₁2₁2₁ with unit cell parameters a=52.8, b=86.8, c=116.4 Å. 100 datasets were merged at 2.6 Å resolution in the published result (Miyauchi et al. Nature Communications, 2017; PDB code: 5Y50) using KAMO; see processing note https://github.com/keitaroyam/yamtbx/wiki/Processing-AtDTX14-data-(5Y50) Note that most frames have lipid rings.多药与有毒化合物外排转运蛋白（Multidrug And Toxic compound Extrusion, MATE）通过利用氢离子（H⁺）梯度外排异生物质。该晶体通过脂立方相法制备获得。 共计288+85（自动+手动）套小楔角（5~20°/晶体）衍射数据集，采集自挂环收集的微晶体。实验于日本同步辐射装置SPring-8的BL32XU线站完成，使用MX225HS型CCD探测器，入射光波长为1 Å。该晶体所属空间群为P2₁2₁2₁，晶胞参数为a=52.8、b=86.8、c=116.4 Å。 已发表结果中，研究人员使用KAMO软件将100套数据集合并至2.6 Å分辨率（Miyauchi等，《Nature Communications》，2017；PDB编号：5Y50）；数据处理说明详见：https://github.com/keitaroyam/yamtbx/wiki/Processing-AtDTX14-data-(5Y50) 请注意：多数衍射帧中存在脂环伪影。