Observation of the effect of bone marrow mesenchymal stem cell transplantation by different interventions on cirrhotic rats

Bone marrow mesenchymal stem cells (BMSCs) transplantation has attracted attention for the treatment of liver cirrhosis and end-stage liver diseases. Therefore, in this study, we evaluated the effect of different methods of BMSCs transplantation in the treatment of liver cirrhosis in rats. Seventy-two male Sprague-Dawley rats were divided into 7 groups: 10 were used to extract BMSCs, 10 were used as normal group, and the remaining 52 rats were randomly divided into five groups for testing: control group, BMSCs group, BMSCs+granulocyte colony-stimulating factor (G-CSF) group, and BMSCs+Jisheng Shenqi decoction (JSSQ) group. After the end of the intervention course, liver tissue sections of rats were subjected to hematoxylin and eosin (H&E) and Masson staining, and pathological grades were scored. Liver function [aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB)] and hepatic fibrosis markers [hyaluronidase (HA), laminin (LN), type III procollagen (PCIII), type IV collagen (CIV)] were measured. BMSCs+JSSQ group had the best effect of reducing ALT and increasing ALB after intervention therapy (P0.05). The effect of JSSQ on improving stem cell transplantation in rats with liver cirrhosis was confirmed. JSSQ combined with BMSCs could significantly improve liver function and liver pathology scores of rats with liver cirrhosis.骨髓间充质干细胞（Bone marrow mesenchymal stem cells, BMSCs）移植在肝硬化及终末期肝病的治疗领域已受到广泛关注。本研究旨在评估不同BMSCs移植方式对大鼠肝硬化的治疗效果。实验共纳入72只雄性斯普拉-道来大鼠，分为7组：10只用于提取BMSCs，10只作为正常对照组，剩余52只大鼠被随机分为5组开展实验：对照组、BMSCs组、BMSCs联合粒细胞集落刺激因子（granulocyte colony-stimulating factor, G-CSF）组、BMSCs联合济生肾气汤（Jisheng Shenqi decoction, JSSQ）组。干预疗程结束后，对各组大鼠的肝组织切片进行苏木精-伊红染色（hematoxylin and eosin, H&E）与Masson染色（Masson staining），并开展病理分级评分；同时检测肝功能指标[谷丙转氨酶（aminotransferase, ALT）、谷草转氨酶（aspartate aminotransferase, AST）、白蛋白（albumin, ALB）]及肝纤维化标志物[透明质酸酶（hyaluronidase, HA）、层粘连蛋白（laminin, LN）、III型前胶原（type III procollagen, PCIII）、IV型胶原（type IV collagen, CIV）]。结果显示，BMSCs联合JSSQ组在干预治疗后降低ALT及升高ALB的效果最为显著（P0.05）。本研究证实，济生肾气汤可有效改善肝硬化大鼠干细胞移植的治疗效果，且BMSCs联合济生肾气汤能够显著改善肝硬化大鼠的肝功能及肝脏病理评分。