<b>Fig. 5.</b> Schematic for a DNA sensor based on quantum do labeled hybridization assay. (a) The capture probe is modified with biotin and reporter with Cy5 fluorescent label. The capture and reporter probe hybridize with target DNA. (b) The complex binds to streptavidin modified quantum dots. The complex is illuminated with light (488 nm) that induces photoluminescence and FRET of the QD and Cy5 respectively. (c) The different wavelength emitted light is detected via a microfluid-type device with illumination using an argon laser (488 nm) using duel photodiodes.

<b>Fig. 5.</b> Schematic for a DNA sensor based on quantum do labeled hybridization assay. (a) The capture probe is modified with biotin and reporter with Cy5 fluorescent label. The capture and reporter probe hybridize with target DNA. (b) The complex binds to streptavidin modified quantum dots. The complex is illuminated with light (488 nm) that induces photoluminescence and FRET of the QD and Cy5 respectively. (c) The different wavelength emitted light is detected via a microfluid-type device with illumination using an argon laser (488 nm) using duel photodiodes.**图5** 基于量子点（quantum dot, QD）标记杂交检测的DNA传感器示意图。(a) 捕获探针经生物素修饰，报告探针带有Cy5荧光标记；捕获探针与报告探针均可与靶DNA发生杂交。(b) 该杂交复合物与链霉亲和素修饰的量子点结合；采用488 nm波长的光照射该复合物，分别诱导量子点产生光致发光，以及Cy5发生荧光共振能量转移（Fluorescence Resonance Energy Transfer, FRET）。(c) 通过搭载488 nm氩离子激光激发的微流控型装置，利用双路光电二极管检测不同波长的发射光。