Genetic basis of transcriptome diversity in Drosophila melanogaster. Drosophila melanogaster

This project uses pooled poly A (+) RNA from the DGRP to derive transcript models specific to DGRP. The derived transcript models provide the basis for quantifying gene expression in the DGRP lines using genome tiling arrays. Overall design: Pooled whole body poly A (+) RNA for females and males of the DGRP lines were barcoded, multiplexed, and sequenced on a single HiSeq 2000 lane using 100PE sequencing