Supplementary Table 2 TRB clones

Ten Eleven Translocation (TET) proteins can oxidize 5-methylcytosine to generate in sequential steps oxidized forms of cytosine: 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxylcytosine. Through their catalytic activity TET proteins promote active DNA demethylation. There are three TET proteins: TET1, TET2 and TET3. In T cells, TET2 and TET3 are more highly expressed. In the past years we have extensively analyzed the impact of TET proteins and 5-hydroxymethylcytosine in T cell development. In this report, we focus on the impact of TET proteins in the TCR alpha (a) and beta (b) repertoires in thymic CD4 single positive cells and upon migration in the periphery. Our data reveal that both wild type and Tet2/3 DKO CD4 cells in the thymus and the spleen are polyclonal. Then, we focus on Tet2/3 DKO CD4 cells that are serially transplanted in recipient mice. Our TCR sequencing data reveals that expanded Tet2/3 DKO CD4 cells are less diverse and oligoclonal. Overall, this report serves as a resource of TCRa and TCRb repertoire in both wild type and Tet2/3 DKO murine conventional CD4 T cells and provides insights on how expanded Tet2/3 DKO CD4 cells opt for specific TCRa and b repertoires.十-十一易位（Ten Eleven Translocation, TET）蛋白可将5-甲基胞嘧啶逐步氧化，依次生成胞嘧啶的三种氧化修饰产物：5-羟甲基胞嘧啶、5-甲酰基胞嘧啶与5-羧基胞嘧啶。TET蛋白通过催化活性介导主动DNA去甲基化过程。目前已发现三类TET蛋白，分别为TET1、TET2与TET3。在T细胞中，TET2与TET3的表达水平更高。过往研究中，我们已针对TET蛋白与5-羟甲基胞嘧啶在T细胞发育过程中的作用开展了系统性分析。本研究聚焦于TET蛋白对胸腺CD4单阳性细胞以及外周迁移过程中T细胞受体α（TCRα）、β（TCRβ）库的调控作用。本研究数据显示，胸腺与脾脏内的野生型及Tet2/3双基因敲除（double knockout, DKO）CD4阳性T细胞均呈多克隆特性。随后，我们针对经受体小鼠连续移植的Tet2/3双基因敲除CD4阳性T细胞展开分析。TCR测序结果表明，体外扩增的Tet2/3双基因敲除CD4阳性T细胞多样性降低，呈现寡克隆特征。综上，本研究提供了野生型与Tet2/3双基因敲除小鼠经典CD4阳性T细胞的TCRα、TCRβ库相关数据资源，并为阐明扩增后的Tet2/3双基因敲除CD4阳性T细胞如何选择特异性TCRα、β链库提供了关键研究依据。