2023_ Datasets and R source code of "Effect of physiological hyperthermia on mitochondrial fuel selection in skeletal muscle of birds and mammals"

GENERAL INFORMATION

Title of Dataset: 2023_ Datasets and R source code of "Effect of physiological hyperthermia on mitochondrial fuel selection in skeletal muscle of birds and mammals" 

METHODOLOGICAL INFORMATION

Datasets contain mitochondrial bioenergetic data of our comparative study from the skeletal muscle, of 8 pigeons and 8 rats with similar body mass.
Methodology: mitochondrial isolation, respiration (oxygen consumption measurements with assay temperature and substrate effects)

## Description of the Data 
First dataset "RatPigeon_Oxy_Flux"
### Individual: subject number (1-8 for pigeons and 11-18 for rats)
### Species: Rat or Pigeon
### Temperature : assay temperature for the mitochondrial respiration(37°C, 40°C, 43°C)
### Substrate: available substrate utilization, Pyruvate/Malate (PM) or PalmitoylCarnitine/Malate (PCM)
### OXPHOS: phosphorylating respiration 
### LEAK: basal non-phosphorylating respiration rate 
### Coupling: control efficiency, flux control of ADP on substrate oxidation

Second dataset "RatPigeon_Oxy_Ratio"
### Individual: subject number (1-8 for pigeons and 11-18 for rats)
### Species: Rat or Pigeon
### Temperature : assay temperature for the mitochondrial respiration(37°C, 40°C, 43°C)
### PCM.PM: fuel selection index (OXPHOSPCM/OXPHOSPM ratio). 

R Source code "code RatPigeon(Oxy).R". Complete analysis as one single R script. All analyses were performed in R version 4.2.1 (R Core Team 2022) using  Linear Mixed Effect Model and Effect sizes.