Protocols for dry DNA storage and shipment at room temperature

The globalization of DNA barcoding will require core analytical facilities to develop cost-effective, efficient protocols for the shipment and archival storage of DNA extracts and PCR products. We evaluated three dry-state DNA stabilization systems: commercial Biomatrica® DNAstable® plates, home-made trehalose and polyvinyl alcohol (PVA) plates on 96-well panels of insect DNA stored at 56 °C and at room temperature. Controls included unprotected samples that were stored dry at room temperature and at 56 °C, and diluted samples held at 4 °C and at −20 °C. PCR and selective sequencing were performed over a 4-year interval to test the condition of DNA extracts. Biomatrica® provided better protection of DNA at 56 °C and at room temperature than trehalose and PVA, especially for diluted samples. PVA was the second best protectant after Biomatrica® at room temperature, whereas trehalose was the second best protectant at 56 °C. In spite of lower PCR success, the DNA stored at −20 °C yielded lo...

DNA条形码(DNA barcoding)技术的全球化应用，要求核心分析平台开发兼具成本效益与运行效率的标准化流程，以实现DNA提取物与聚合酶链式反应(PCR)产物的运输及归档存储。我们针对在56℃与室温条件下存储的昆虫DNA 96孔板样本，评估了三种干态DNA稳定体系：商业化Biomatrica® DNAstable® 板，以及自制海藻糖板与聚乙烯醇(PVA)板。对照组设置包括：分别在室温与56℃下干燥存储的未保护样本，以及4℃与-20℃下保存的稀释样本。研究在为期4年的周期内分段开展PCR扩增与选择性测序，以检测DNA提取物的降解状态。相较于海藻糖与聚乙烯醇(PVA)体系，Biomatrica® 体系对56℃及室温条件下存储的DNA具有更优异的保护效果，针对稀释样本的保护作用尤为显著。在室温条件下，聚乙烯醇(PVA)是仅次于Biomatrica®的优质稳定剂；而在56℃条件下，海藻糖的保护效果位列第二。尽管PCR扩增成功率较低，但-20℃下存储的DNA仍能获得……