Analysis of organic molecules, physicochemical parameters, and pollen as indicators for authenticity, botanical origin, type and quality of honey samples examined

To contribute to Chilean honey’s characterization, 12 honey samples were analyzed using comprehensive physicochemical and pollen analyses. Beekeepers donated samples from La Pintana, Linderos, Cajón del Maipo, and Chiloé. Physicochemical parameters required for honey authentication such as free acidity (in range of 9.5–46 meq/kg), hydroxymethylfurfural (0–8 mg/kg), humidity (14.4–16.9%), sugar profile, amino acid profile, organic acid profile, pH (3.8–4.7), electrical conductivity (0.25–1.47 mS/cm) and diastase activity (28.6–43.8 ºG), were determined by conventional techniques and nuclear magnetic resonance (NMR) at an international quality control laboratory for honey analyses. The Chilean honey samples analyzed showed physicochemical properties in normal ranges and typical sugar profiles for natural honey, which confirmed their authenticity and high quality.

为推进智利蜂蜜的特性表征研究，本研究采用综合理化分析与花粉分析方法，对12份蜂蜜样品进行了检测。所分析的样品由养蜂人从拉平塔纳（La Pintana）、林德罗斯（Linderos）、迈波峡谷（Cajón del Maipo）及奇洛埃（Chiloé）地区捐赠提供。蜂蜜真伪鉴定所需的各项理化参数，包括游离酸度（范围9.5~46 meq/kg）、羟甲基糠醛（0~8 mg/kg）、水分含量（14.4%~16.9%）、糖谱、氨基酸谱、有机酸谱、pH值（3.8~4.7）、电导率（0.25~1.47 mS/cm）及淀粉酶活性（28.6~43.8 ºG），均在一家专业从事蜂蜜检测的国际质量控制实验室，通过常规检测技术与核磁共振（NMR）完成测定。经检测的智利蜂蜜样品，其理化性质均处于正常范围，糖谱也符合天然蜂蜜的典型特征，证实了这些样品的真实性与高品质。