DNA-seq or genomic RNA region of HIV-1 reverse transcriptase, protease and integrase gene

To assess compatibility in sequence analysis we compared results from Sanger sequencing (with sequencing threshold >15%) and Next Generation Sequencing (with sequencing treshold >5%). Totally, there were 48 patients included in this part of the study but sequencing for one sample failed. Here we demonstrate how reliable tool for fast and accurate identification of low-level viral quasispecies is deep-sequencing.