Rapid cell proliferation of hiPSCs plated at low cell density on laminin-521 in Essential 8 medium.

(A-I) Quantification of the number of 253G1, 201B7 and 409B2 cells expanded on laminin-521 in Essential 8 or mTeSR1 medium. Cell numbers were counted every 24 h after plating at 3.2×104 cells/cm2 (A, D, G), 1.6×104 cells/cm2 (B, E, H) and 8.0×103 cells/cm2 (C, F, I), respectively. Data are presented as the mean ± standard deviation (SD) of three independent experiments (*PPP(A-I) 对在层粘连蛋白-521（laminin-521）包被表面上，采用Essential 8或mTeSR1培养基扩增的253G1、201B7及409B2细胞的数量进行定量分析。分别以3.2×10⁴ 个细胞/平方厘米（A、D、G）、1.6×10⁴ 个细胞/平方厘米（B、E、H）及8.0×10³ 个细胞/平方厘米（C、F、I）的密度铺板接种后，每24小时计数一次细胞数目。数据以三次独立实验的平均值±标准差（standard deviation, SD）表示（*PPP