Protective effects of Ginger Essential Oil (GEO) against chemically-induced cutaneous inflammation

Abstract The inhibitory effects of ginger essential oil (GEO) on chemically-induced cutaneous inflammation were investigated. Three kind of experimental murine models, such as xylene-induced ear edema model, carrageenan-induced paw edema model and 2,4-dinitrofluorobenzene (DNFB)-induced allergic contact dermatitis (ACD) model were exploited to evaluate the anti-inflammatory effect of GEO. Results showed that GEO lowered the ear swelling rate induced by xylene and decreased paw edema rate induced by carrageenan of mice with cutaneous inflammation significantly. Treatment with GEO significantly inhibited DNFB-induced ear swelling degree and rate, and alleviated the inflammatory symptoms of ear skin lesions of ACD model mice markedly. Treatment with GEO decreased the gene expression of the inflammatory cytokines of auricular tissues such as TLR-2, TLR-4, TNF-α, IFN-γ, IL-1β, IL-8, and increased the gene expression of cytokine IL-4 significantly. In conclusion, GEO has anti-inflammatory and protective effect on chemically-induced cutaneous inflammation.

摘要
本研究针对生姜精油（Ginger Essential Oil, GEO）对化学诱导性皮肤炎症的抑制效果展开探究。本研究采用三种小鼠实验模型，即二甲苯诱导耳肿胀模型、角叉菜胶诱导足肿胀模型以及2,4-二硝基氟苯（2,4-Dinitrofluorobenzene, DNFB）诱导的变应性接触性皮炎（Allergic Contact Dermatitis, ACD）模型，用以评估GEO的抗炎活性。实验结果显示，GEO可显著降低皮肤炎症模型小鼠由二甲苯诱导的耳肿胀率，以及由角叉菜胶诱导的足肿胀率。GEO干预可显著抑制DNFB诱导的耳肿胀程度与肿胀率，并显著缓解ACD模型小鼠耳部皮肤病变的炎症症状。此外，GEO干预可下调耳部组织中TLR-2、TLR-4、TNF-α、IFN-γ、IL-1β、IL-8等炎性细胞因子的基因表达，并显著上调细胞因子IL-4的基因表达。综上，GEO对化学诱导性皮肤炎症具有抗炎与保护作用。