Additional file 10 of Detection and discovery of plant viruses in soybean by metagenomic sequencing

Additional file 10 Figure S10. Mixed infections of viruses confirmed by Sanger sequencing in field-grown soybean plants. Reverse transcription-polymerase chain reaction (RT-PCR) was performed in all samples identified with mixed infections. The PCR products were visualized in a 1% agarose gel electrophoresis containing SYBR Safe DNA gel stain. The PCR products were cleaned using ExoSAP-IT PCR product cleanup reagent and sequenced on Sanger sequencing platform. Primer sequences were designed using Primer3 plus from assembled contigs and subsequently used in this study补充材料10 图S10：田间种植大豆植株中经桑格（Sanger）测序验证的病毒混合侵染状况。针对所有鉴定为病毒混合侵染的样本，均实施了逆转录聚合酶链式反应（RT-PCR）扩增。将PCR产物置于添加了SYBR Safe DNA凝胶染料的1%琼脂糖凝胶中进行电泳可视化检测。采用ExoSAP-IT PCR产物纯化试剂对上述PCR产物进行纯化后，通过桑格测序平台完成测序。本研究基于组装得到的重叠群（contigs），通过Primer3 plus工具设计引物序列，并将其应用于本项实验。