BMT decreases HFD-induced weight gain associated with decreased preadipocyte number and insulin secretion

Experimental bone marrow transplantation (BMT) in mice is commonly used to assess the role of immune cell-specific genes in various pathophysiological settings. The application of BMT in obesity research is hampered by the significant reduction in high-fat diet (HFD)-induced obesity. We set out to characterize metabolic tissues that may be affected by the BMT procedure and impair the HFD-induced response. Male C57BL/6 mice underwent syngeneic BMT using lethal irradiation. After a recovery period of 8 weeks they were fed a low-fat diet (LFD) or HFD for 16 weeks. HFD-induced obesity was reduced in mice after BMT as compared to HFD-fed control mice, characterized by both a reduced fat (-33%; p小鼠骨髓移植（bone marrow transplantation, BMT）实验常被用于评估免疫细胞特异性基因在各类病理生理环境中的作用。然而骨髓移植在肥胖研究中的应用却受到阻碍，原因是高脂饮食（high-fat diet, HFD）诱导的肥胖现象显著减轻。本研究旨在明确可能受骨髓移植操作影响并削弱高脂饮食诱导机体应答的代谢组织。研究对象为雄性C57BL/6小鼠，通过致死性辐照构建同基因骨髓移植模型。经过8周的恢复周期后，将小鼠分为低脂饮食（low-fat diet, LFD）组与高脂饮食组，持续喂养16周。与高脂饮食喂养的对照组小鼠相比，骨髓移植后的小鼠高脂饮食诱导的肥胖程度有所减轻，表现为体脂含量降低（-33%；P