Source data for: Effect of seed length and binding motifs on Hfq-mediated sRNA-mRNA annealing analyzed using single-molecule FRET

This dataset contains raw and processed single-molecule FRET (smFRET) data used to quantify Hfq-mediated annealing between bacterial sRNAs and mRNAs. It includes fluorescence time traces, extracted dwell times, FRET distributions, and analysis scripts supporting measurements of how seed length, Hfq-binding motifs, and motif spacing affect interaction stability and kinetics. The data enable independent reproduction of the analyses and reuse for modeling RNA–RNA interaction dynamics and RNA chaperone function. Funding: This research was funded by the National Science Centre, Poland, grant no 2022/46/E/NZ1/00462 and an EMBO Installation Grant IG 5730-2024.

本数据集包含用于定量细菌小RNA（sRNAs）与信使RNA（mRNA）之间Hfq介导退火反应的原始及处理后单分子荧光共振能量转移（single-molecule FRET, smFRET）数据。数据集涵盖荧光时间轨迹、提取得到的停留时间、FRET分布，以及支持相关测量的分析脚本，这些测量用于探究种子序列长度、Hfq结合基序及其间隔距离如何影响互作稳定性与动力学特性。本数据集可支持分析结果的独立复现，亦能复用至RNA-RNA互作动力学及RNA分子伴侣功能的建模研究中。资助说明：本研究受波兰国家科学中心资助（项目编号：2022/46/E/NZ1/00462）以及欧洲分子生物学组织（EMBO）安装项目资助金IG 5730-2024。