Recordings from periaqueductal gray/dorsal raphe in adult female rats that underwent early adolescent adversity

These data were collected from the periaqueductal gray/dorsal raphe of 20 female Long Evans rats using 16 wire drivable microelectrode bundles. Data include 151 single units in 8 Control rats over 72 fear discrimination sessions, and 203 single units in 12 Early Adolescent Adversity (EAA) rats over 107 fear discrimination sessions. Fear discrimination sessions consisted of 32 trials with a mean inter-trial interval of 3.5 min. Auditory cues were 10 s in duration and consisted of repeating motifs of a broadband click, phaser, or trumpet. Each cue was associated with a unique probability of foot shock (0.5 mA, 0.5 s): danger, p = 1.00; uncertainty, p = 0.375; and safety, p = 0.00. Auditory identity was counterbalanced across rats. Foot shock was administered 2 s following the termination of the auditory cue on danger and uncertainty shock trials. The order of trial type presentation was randomly determined by the behavioral program, and differed for each rat, each session. The microelectrode bundle was advanced in ~84 μm steps every other day to record from new units during the following session. For six of the twenty rats, the electrode bundle was centered at -7.68 mm from bregma, for seven of the twenty rats, the electrode bundle was centered at -7.92 mm from bregma, for five of the twenty rats the electrode bundle was centered at -8.16 mm from bregma, and the other two rats had electrodes centered at -8.40 mm from bregma. Data included are the result of spike sorting. Sample scripts used to visualize firing and behavior data from our main data file have been provided. These data have been published in Scientific Reports (reference below) and the complete methods are available with that publication. Moaddab, M., Wright, K.M. & McDannald, M.A. Early adolescent adversity alters periaqueductal gray/dorsal raphe threat responding in adult female rats. Sci Rep 10, 18035 (2020). https://doi.org/10.1038/s41598-020-74457-3