Optimizing the use of in vitro Transcribed SGK1-mRNA as a therapeutic tool to treat female infertility (Sanger sequencing ABI files)

SGK1 plasmids were commercially purchased from MRC PPU Dundee, UK and have been previously characterised for their function. SGK1 and its variants were subcloned into the pVAX.A120 vector containing, a poly(A)-120 tail using sticky-end ligation. The clones were then subject to sanger sequencing to confirm the assmembly and their mutational status. The files contained in this folder contain the raw sequencing files.SGK1质粒（SGK1 plasmids）购自英国邓迪的MRC PPU，其功能已在既往研究中完成表征。研究人员采用粘性末端连接法，将SGK1及其变体亚克隆至带有poly(A)-120尾（poly(A)-120 tail）的pVAX.A120载体（pVAX.A120 vector）中。随后对重组克隆开展桑格测序（Sanger sequencing），以验证克隆组装的正确性与突变状态。 本文件夹内收录的文件均为原始测序数据文件。