Functional enrichment of genes preferentially expressed in peels at 16 days post pollination (dpp).

a Functional distribution, normalized frequency, and bootstrap standard deviation (SD) of contigs with putative Arabidopsis homologs was determined using the categories classification from the Classification SuperViewer from Bio-Array Resource for Arabidopsis Functional Genomics for Gene Ontology [http://compbio.dfci.harvard.edu/tgi/982 software96/].Transcripts were selected based on criteria of top 5% for expression in peel and top 5% for increase in 16 dpp peel vs. 8 dpp peel. The resulting 105 transcripts had >72% of reads in peel samples and >8-fold increase above 8 dpp peel samples.本研究采用面向基因本体（Gene Ontology）的拟南芥功能基因组学生物阵列资源库（Bio-Array Resource for Arabidopsis Functional Genomics）的分类超级查看器（Classification SuperViewer）所提供的分类体系，对携带推定拟南芥同源基因的重叠群（contigs）的功能分布、归一化频率及自助法标准差（bootstrap standard deviation, SD）进行了统计计算。转录本的筛选标准为：在果皮组织中表达量排名前5%，且授粉后16天（16 dpp）果皮相较于授粉后8天（8 dpp）果皮的表达量上调幅度同样位列前5%。最终筛选得到的105条转录本，在果皮样本中的测序读段占比超过72%，且相较于授粉后8天果皮样本的表达量上调幅度超过8倍。[http://compbio.dfci.harvard.edu/tgi/982 software96/]