Illumina MiSeq sequenced 16S library of Microbial Community Response to Oil and Corexit in Coastal Water Mesocosms

Two independent mesocosm experiments were carried out in August (Mesocosm#1) and October of 2015 (Mesocosm#2) using coastal water collected near Galveston Bay, Texas. Four mesocosm treatments were prepared in triplicate for this study: (1) Control (seawater only); (2) WAF (oil + seawater); (3) Chemically-enhanced WAF (CE-WAF; oil + Corexit + seawater); (4) Diluted CE-WAF (1:10 dilution). Initial mesocosm volumes were 79L. Prior to experiment initiation, phytoplankton were collected from coastal Galveston, TX waters and added to each tank to stimulate interactions between phytoplankton, oil, and Corexit. Starting at time-point zero and every 12 hours thereafter, water was collected from each mesocosm in clean, opaque Nalgene bottles with PTFE-lined diaphragm pumps. DNA was extracted from filtered water samples and sequenced using barcoded primers specific for the V4 hypervariable region of the 16S rRNA gene.

本研究于2015年8月（中型实验生态系#1）及2015年10月（中型实验生态系#2）开展两项独立的中型实验生态系（mesocosm）实验，实验所用海水采自美国得克萨斯州加尔维斯顿湾附近沿岸海域。本研究共设置4组中型实验生态系处理组，每组均设置三次生物学重复：(1) 对照组（仅天然海水）；(2) 水包油乳化液组（WAF，原油+天然海水）；(3) 化学强化型水包油乳化液组（CE-WAF，原油+Corexit+天然海水）；(4) 稀释型CE-WAF组（按1:10比例稀释的CE-WAF）。每组中型实验生态系初始容积为79升。实验启动前，研究人员从得克萨斯州加尔维斯顿沿岸海域采集浮游植物，并接种至各实验罐，以模拟浮游植物、原油与Corexit之间的相互作用。自实验零时起，后续每间隔12小时，研究人员使用内衬聚四氟乙烯（PTFE）的隔膜泵，从每个中型实验生态系中采集海水样品，保存于洁净的不透明耐洁（Nalgene）样品瓶中。对过滤后的水样提取基因组DNA，并采用靶向16S rRNA基因V4高变区的带条码引物进行测序。