ST4: Number of differentially expressed genes for various contrasts

Human lung adenocarcinoma cells (A549) and human monocytic cells (THP-1) were infected with H3N23 influenza virus A virus. Transcriptome analysis were performed by next generation RNA sequencing of three replicates each for infected A549 cells at 4, 8 and 24h p.i. and infected THP-1 cells at 4, 8, 12 and 24h p.i., and corresponding set of non-infected controls. Using DESeq2 (version 2.7.9a, (45)) with thresholds log2=1 for log-fold change and adjusted p-value of > 0.05, we identified many differentially expressed genes (DEGs) for various contrasts. The number of differentially expressed genes for various contrasts are shown here.本研究将人肺腺癌细胞（A549）与人类单核细胞（THP-1）感染H3N23亚型甲型流感病毒。分别针对感染后（post infection, p.i.）4、8、24小时的A549感染细胞、感染后4、8、12、24小时的THP-1感染细胞各设置三次生物学重复，并同步设置对应的非感染对照组，随后通过下一代RNA测序（next generation RNA sequencing）开展转录组分析。本研究使用DESeq2（版本2.7.9a，参考文献45），以log2倍数变化≥1、校正后P值>0.05作为筛选阈值，针对多种比较组合鉴定出大量差异表达基因（differentially expressed genes, DEGs）。各类比较组合对应的差异表达基因数量如下所示。