Microarray expression analysis of patients Dupuytren´s contracture (DC)

We used a high-throughput technology, DNA microarray, to screen the entire genome for the changes in gene expression in diseased tissue to characterize Dupuytren's contracture at a molecular level and find genes that are involved in development of the disease. Primary cell cultures were established from surgically removed diseased (D) and healthy (ND) tissue from 3 patients. Total we had 6 samples, 3 diseased and 3 healthy patient-matched samples. Total RNA was isolated by RNeasy mini kit (Qiagen) and further used for microarray expression analysis (HG-U133A array, Affymetrix). Raw data, CEL. files were analysed with software MAS5, GeneSpring and GENEMAPP.