Intraspecies variability effect in the identification of targets and possible offtargets.

For each example, upper file shows the targets and offtargets listed by ARES-GT (with thresholds L0 = 4 and L1 = 3) for each reference genome. SNPs differences between genomes that explain why some targets or offtargets are not detected are shown in lower file (separated by discontinuous line) as red boxes. Black boxes mark mismatches with candidates sequence.针对每个示例，上方文件展示了由ARES-GT针对各参考基因组所列出的靶位点与脱靶位点（阈值设置为L0=4、L1=3）。下方文件以间断线分隔内容，其中以红色框标注了基因组间的单核苷酸多态性（Single Nucleotide Polymorphisms, SNPs）差异——此类差异可解释为何部分靶位点或脱靶位点未被成功检测；黑色框则用于标记与候选序列存在错配的位点。