Raw data-image analysis.

IntroductionMultiple sclerosis is a chronic demyelinating disease of the central nervous system. Transplantation of oligodendrocyte progenitor cells (OPCs) is a promising approach to enhance remyelination; however, the influence of the OPCs’ microenvironmental origin on their therapeutic efficacy remains unclear. This study compared the remyelinating capacity of OPCs isolated from inflammatory (lipopolysaccharide) and non-inflammatory (cuprizone) microenvironments after transplanting into the corpus callosum and examined their effects on extracellular matrix chondroitin sulfate proteoglycans (CSPGs).MethodsOPCs were isolated from two microenvironments and characterized by immunocytochemistry and RT-qPCR. After transplanting, OPC homing, remyelination, gene expression, and CSPG levels were evaluated using DiI labeling, LFB staining, RT-qPCR, and immunofluorescence, respectively.ResultsSevere demyelination exhibited in the cuprizone group compared with healthy controls (p ConclusionOPC transplantation improves remyelination and reduces the CSPG level, but the effectiveness is more related to the previous history of the OPC isolation microenvironment and the new donor.引言：多发性硬化（Multiple sclerosis）是一种中枢神经系统慢性脱髓鞘疾病。少突胶质细胞祖细胞（oligodendrocyte progenitor cells, OPCs）移植是促进髓鞘再生的极具前景的治疗策略，但OPCs的微环境起源对其治疗效能的影响仍不明确。本研究对比了从炎性（脂多糖，lipopolysaccharide）微环境与非炎性（双环己酮草酰二腙，cuprizone）微环境中分离的OPCs经胼胝体移植后的髓鞘再生能力，并检测了其对细胞外基质硫酸软骨素蛋白聚糖（chondroitin sulfate proteoglycans, CSPGs）的影响。 方法：从两种微环境中分离OPCs，并通过免疫细胞化学技术与实时定量聚合酶链式反应（RT-qPCR）对其进行鉴定。移植后，分别采用DiI标记、LFB染色、RT-qPCR与免疫荧光技术，评估OPCs的归巢能力、髓鞘再生情况、基因表达水平以及CSPG含量。 结果：与健康对照组相比，cuprizone组呈现严重脱髓鞘表现（p 结论：OPC移植可促进髓鞘再生并降低CSPG水平，但其治疗效果更与OPCs分离时的微环境既往史以及新供体相关。