NanoSINC-seq
收藏NIAID Data Ecosystem2026-03-12 收录
下载链接：
https://www.ncbi.nlm.nih.gov/sra/DRP007143
下载链接
链接失效反馈官方服务：
资源简介：
Transcripts are synthesized in the nucleus and then exported to the cytoplasm, where translation occurs. Alternative isoform usage in the cytoplasm is essential to diversify the protein molecules even in isogenic populations of cells. To obtain a comprehensive understanding of isoform localization and the usage, we here leveraged the physical fractionation of cytoplasmic RNAs versus nucleus via a microfluidic approach coupling with Oxford Nanopore Technologies (ONT) long-read sequencing (NanoSINC-seq). Leveraging the NanoSINC-seq approach, we investigated the difference in transcript usage between nucleus and cytoplasm in leukemic cells (K562).
创建时间：
2021-04-09
