aFrequency with which candidate colonies were successfully verified.bNumber of colonies/number of viable cells.cRelative efficiency of FRUIT as compared to pKD13.

Figshare2015-12-02 更新40

Staphylococcus epidermidis RP62a CRISPR tall effector complex with bound ATP

CRISPR效应蛋白

细菌免疫机制

Staphylococcus epidermidis RP62a CRISPR tall effector complex with bound ATP

NIAID Data Ecosystem20

Escherichia coli strain:K-12 DH10B Raw sequence reads. Escherichia coli strain:K-12 DH10B

细菌基因编辑

CRISPRPrimeEditing

Illumina data for CRISPR Prime Editing, a Cas9 nickase-reverse transcriptase assisted versatile genetic engineering toolkit for E. coli

NIAID Data Ecosystem60

An rpsL Cassette, Janus, for Gene Replacement through Negative Selection in Streptococcus pneumoniae

细菌基因编辑

负选择标记系统

Natural genetic transformation offers a direct route by which synthetic gene constructs can be placed into the single circular chromosome of Streptococcus pneumoniae. However, the lack of a general ne

PubMed Central20