Energy strategies of brown algae Lessonia spicata (Surh) Santelices (Phaeophyceae, Laminariales) to counter excess solar irradiance in the intertidal zone

This dataset contains the physiological, biochemical, and molecular measurements obtained from the intertidal brown macroalga Lessonia spicata exposed to simulated seasonal irradiance regimes under controlled laboratory conditions. The experiment evaluated short-term acclimation responses to different combinations of photosynthetically active radiation (PAR) and ultraviolet radiation (UVA + UVB), representing seasonal light conditions (autumn, winter, spring, and summer) typical of the central Chilean coast.

The dataset is organized into three sheets:

Physiological responses: chlorophyll a fluorescence parameters derived from PAM fluorometry, including photosynthetic efficiency (αETR), maximum electron transport rate (ETRmax), saturation irradiance (Ek), maximum quantum yield of PSII (Fv/Fm), and maximum non-photochemical quenching (NPQmax).

Biochemical responses: pigment concentrations (chlorophyll a), phenolic compounds, and oxidative stress indicators including hydrogen peroxide (H₂O₂) and lipid peroxidation (TBARS/MDA).

Molecular responses: raw quantitative PCR values associated with genes involved in photosynthesis, pigment biosynthesis, electron transport and stress response.

Samples were obtained from adult sporophytes of Lessonia spicata collected in the intertidal zone of central Chile and analyzed after exposure to controlled irradiance treatments. Measurements were conducted during a daily cycle at multiple sampling times to capture short-term physiological and transcriptional responses.

本数据集包含在受控实验室条件下，经模拟季节光照制度处理的潮间带大型褐藻刺状昆布（Lessonia spicata）的生理、生化与分子检测数据。本实验评估了该藻类对光合有效辐射（Photosynthetically Active Radiation, PAR）与紫外辐射（UVA + UVB）不同组合的短期驯化响应，这些组合对应智利中部海岸典型的春、夏、秋、冬四季光照条件。

本数据集分为三个工作表：
1. 生理响应工作表：包含由脉冲振幅调制（Pulse Amplitude Modulation, PAM）荧光法测得的叶绿素a荧光参数，具体包括光合效率（αETR）、最大电子传递速率（ETRmax）、饱和光照强度（Ek）、PSII最大量子产率（Fv/Fm）以及最大非光化学淬灭（NPQmax）。
2. 生化响应工作表：包含色素浓度（叶绿素a）、酚类化合物，以及氧化应激指标，其中氧化应激指标包括过氧化氢（H₂O₂）与脂质过氧化产物（TBARS/MDA）。
3. 分子响应工作表：包含与光合作用、色素生物合成、电子传递及应激响应相关基因的原始定量PCR数值。

实验样本采自智利中部潮间带的成年刺状昆布（Lessonia spicata）孢子体，经受控光照处理后开展检测。本研究在每日周期内设置多个采样时间点进行检测，以捕捉短期生理与转录响应。