Comparative analysis of 43 distinct RNA modifications by nanopore tRNA sequencing - RNA004 data

This project focuses on nanopore direct tRNA sequencing method refinement and benchmarking of RNA modification detection between Oxford Nanopore's previous direct RNA sequencing chemistry, RNA002, and the RNA004 chemistry released in November 2023. The majority of the data was collected during a December 2023 tRNA sequencing workshop sponsored by the Hesselberth lab at the University of Colorado, in which participants prepared matched sequencing libraries using both chemistries from tRNA isolated from six different species. Fastq data has been submitted to the SRA (GSE272876); here we are hosting the raw POD5 data from all libraries prepared with RNA004 chemistry as well as select libraries prepared with the deprecated RNA002 chemistry for signal reanalysis.本项目聚焦于纳米孔直接转运RNA（tRNA）测序方法的优化改进，并针对牛津纳米孔（Oxford Nanopore）前代直接RNA测序试剂套件RNA002与2023年11月发布的RNA004试剂套件的RNA修饰检测性能开展基准评测。本项目的绝大多数数据采集自2023年12月由科罗拉多大学赫塞尔伯思（Hesselberth）实验室主办的tRNA测序研讨会，与会者针对从6个不同物种中提取的tRNA，分别使用两种试剂套件构建匹配的测序文库。 FASTQ格式（Fastq）数据已提交至序列读取档案（Sequence Read Archive, SRA），登录编号为GSE272876；本数据集提供所有采用RNA004试剂套件构建的文库的原始POD5格式数据，以及部分采用已停用的RNA002试剂套件构建的文库的原始数据，用于信号重分析。