Details of primers for target and reference genes analyzed by qPCR

Details of primers for target and reference genes analyzed by qPCR used for cotyledons expression. Primer designs were obtained from Rotta et al. (2015) and from GenBank for the following targets: 18S ribosomal RNA, vascular endothelial growth factor A (VEGFA), vascular endothelial growth factor B (VEGFB), insulin-like growth factor 1 receptor (IGFR1), insulin-like growth factor 2 receptor (IGFR2), guanilate cyclase 1 soluble subunit beta (GUCY1B3), hypoxia inducible factor 1-alpha (HIFA), splicing factor 3a subunit 1 (SF3A1), fibroblast growth factor 2 (FGF2), angiopoietin 2 (ANGPT2), and nitric oxide synthase (NOS3).

本数据集包含用于子叶基因表达分析的实时定量聚合酶链式反应（qPCR）检测所需的靶基因与内参基因引物的详细信息。本次实验所用引物的设计信息来源于Rotta等（2015）的研究成果以及GenBank数据库，涉及的靶基因如下：18S核糖体RNA（18S ribosomal RNA）、血管内皮生长因子A（vascular endothelial growth factor A，VEGFA）、血管内皮生长因子B（vascular endothelial growth factor B，VEGFB）、胰岛素样生长因子1受体（insulin-like growth factor 1 receptor，IGFR1）、胰岛素样生长因子2受体（insulin-like growth factor 2 receptor，IGFR2）、鸟苷酸环化酶1可溶性亚基β（guanilate cyclase 1 soluble subunit beta，GUCY1B3）、缺氧诱导因子1-α（hypoxia inducible factor 1-alpha，HIFA）、剪接因子3a亚基1（splicing factor 3a subunit 1，SF3A1）、成纤维细胞生长因子2（fibroblast growth factor 2，FGF2）、血管生成素2（angiopoietin 2，ANGPT2）以及一氧化氮合酶（nitric oxide synthase，NOS3）。