DADP Excel Database of Anuran Defense Peptides

Look for the DOI: 10.1093/bioinformatics/bts141 paper, deposited today (2025-07-04) on ZENODO, by Davor Juretić, which describes the origin and all entries in the DADP Excel database of anuran defense peptides. Take into account that the database was up-to-date in 2012, but there was no manual curation and addition of discovered anuran defense peptides after that year. Also, functional annotation in the top-located table and figure is not complete. It contains only antibacterial and an unknown function, but column H obviously mentions other functions such as anticancer, antioxidant, and insulin-releasing. That issue was better solved in the corresponding web server located at the Faculty of Science, University of Split, Croatia. Unfortunately, that dedicated scientific web server was discontinued in the spring of 2025. Following this contribution, interested students and researchers can freely utilize the DADP Excel database as a tool to support studies on the evolutionary origin of the rich endowment of bioactive peptides found in the skin of most frogs and toads. It can also be used to help find novel potential AMPs in genomic or EST databases of amphibians by indirect search for the conserved signal peptides, and the presence of the characteristic tripartite sequence arrangement – signal peptide followed by acidic propeptide and mature AMP/bioactive peptide. Reconstructing the DADP web server enriched with natural bioactive peptides from frogs and toads, as found between 2012 and 2025, is also possible, with appropriate credit to the creators of the Excel database, Dr. Mario Novković and Professor Emeritus Davor Juretić.请查阅DOI为10.1093/bioinformatics/bts141的论文，该论文由Davor Juretić发布并于2025年7月4日上传至泽诺多（ZENODO）平台，详细阐述了无尾目防御肽（anuran defense peptides）DADP Excel数据库的构建背景与全部收录条目。需注意，该数据库的最新更新节点为2012年，此后未再开展人工审校与已发现无尾目防御肽的新增工作。此外，数据库顶部表格与图表中的功能注释并不完整：仅标注了抗菌活性与未知功能，但H列明确提及了抗癌、抗氧化与胰岛素释放等其他活性功能。该数据的完整化可依托克罗地亚斯普利特大学理学院对应的专用科学网页服务器实现，但遗憾的是，该专用网页服务器已于2025年春季停运。 基于该数据集，感兴趣的学生与研究人员可免费使用DADP Excel数据库，以此开展多数蛙类与蟾蜍皮肤中丰富生物活性肽的进化起源相关研究。该数据库还可通过间接搜索保守信号肽（signal peptide），并识别特征性三分区序列结构（即信号肽紧随酸性前肽与成熟抗菌肽（Antimicrobial Peptide, AMP）/生物活性肽），辅助在两栖动物基因组或表达序列标签（Expressed Sequence Tag, EST）数据库中挖掘新型潜在抗菌肽。若需重建DADP网页服务器，补充2012年至2025年间发现的蛙类与蟾蜍天然生物活性肽，该方案同样可行，且需注明该Excel数据库的创建者——Mario Novković博士与荣誉退休教授Davor Juretić。