Tansey HNMR Metabolimcs Human Plasma

Plasma samples from IBD, PD and NHC groups were extracted as previously described (Kadyan et al., 2023) to generate a final solution of 10% D2O, 0.1 M phosphate, and 0.1 mM Trimethylsilyl propionate (TSP). Following extraction, samples data was acquired via Bruker Ascend 400 MHz high-resolution NMR and all spectra were phased and referenced to TSP in TopSpin 4.06 (Bruker BioSpin, Germany), as well as all spectra were normalized to total intensity.按照此前已报道的方法（Kadyan等，2023）提取炎症性肠病（IBD）、帕金森病（PD）及正常健康对照（NHC）组的血浆样本，制备得到含10%氘代水（D₂O）、0.1 mol/L磷酸盐缓冲液及0.1 mmol/L三甲基硅基丙酸酯（Trimethylsilyl propionate, TSP）的终溶液。样本提取完成后，采用布鲁克Ascend 400 MHz高分辨率核磁共振波谱仪（NMR）采集样本数据；所有光谱均在德国布鲁克生物自旋（Bruker BioSpin）公司的TopSpin 4.06软件中完成相位校正与以TSP为内标的化学位移定标，并通过总强度归一化处理全部光谱。