Additional file 9 of Transcriptome analyses of liver in newly-hatched chicks during the metabolic perturbation of fasting and re-feeding reveals THRSPA as the key lipogenic transcription factor

Additional file 9: Microarray reference RNA hybridization design. A Microsoft Excel file containing a single worksheet “20.7 K Array Hybridization Design”. A total of 50 Arizona 20.7 K Chicken Oligo Arrays (NCBI GEO Platform # GPL6049) were used in a reference RNA hybridization design. Each biological sample (liver RNA sample) was labelled with Alexa Flour® 555 (green) dye. An aliquot of the pooled reference RNA sample was labelled with Alexa Flour® 647 (red) dye and these were hybridized together on one of the 20.7 K oligo microarrays. The 50 microarrays were assigned to 10 fasting-refeeding treatment groups with five biological replicates (liver RNA from five cockerels). A balanced block design was used for hybridization of 10 microarrays across 5 consecutive days, where one liver RNA sample represented each of the 10 treatment groups on each hybridization day (10 arrays/day).附加文件9：微阵列参考RNA杂交实验设计。本文件为Microsoft Excel格式，仅包含一个名为"20.7 K阵列杂交设计"的工作表。本次参考RNA杂交实验共使用50张亚利桑那20.7K鸡寡核苷酸微阵列（NCBI GEO平台编号GPL6049）。每份生物学样本（肝脏RNA样本）均以Alexa Fluor® 555（绿色）荧光染料标记；将混合后的参考RNA样本的等分试样以Alexa Fluor® 647（红色）荧光染料标记，随后将两种标记样本共同杂交于单张20.7K寡核苷酸微阵列上。50张微阵列被分配至10个禁食-复喂处理组，每组设置5个生物学重复样本（取自5只公鸡的肝脏RNA）。实验采用平衡区组设计：在连续5天的杂交周期内，每日完成10张微阵列的杂交，每个杂交日中，1份肝脏RNA样本对应10个处理组中的1个（即每日杂交10张阵列）。