Methodological details from some recent illustrative studies examining “omics” of Fe limitation on diatoms revealing the wide range of methodological approaches that have been employed.

qRT-PCR = quantitative reverse transcription polymerase chain reaction. 2D SDS PAGE LC/MS/MS = 2 dimensional SDS PAGE gel electrophoresis followed by tandem mass spectrometry protein identifications on individual gel spots.qRT-PCR（定量反转录聚合酶链反应，quantitative reverse transcription polymerase chain reaction）；2D SDS PAGE LC/MS/MS 指先开展二维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（2D SDS PAGE），随后通过串联质谱对单个凝胶斑点进行蛋白质鉴定的技术。