An omics-based metabolic model of methane-driven denitrification by aerobic methanotrophic bacteria

Methylomonas denitrificans is the first characterized organism that couples methane oxidation to nitrate reduction in pure culture without a partner. The aim of this study is to develop a metabolic model of methane driven nitrate respiration using a multi-level omics approach. Samples of M. denitrificans biomass were harvested during growth at high and limiting oxygen and whole transcriptome/proteome analyses were carried out on samples from both conditions. Comparative analysis of transcript and protein abundance between the two conditions was used to develop a metabolic model of methane driven nitrate respiration. 1. Wisniewski JR, Zougman A, Nagaraj N, Mann M: Universal sample preparation method for proteome analysis. Nat Meth 2009, 6:359-362. 2. Oberg AL, Vitek O: Statistical design of quantitative mass spectrometry-based proteomic experiments. J Proteome Res 2009, 8:2144-2156. 3. Olsen JV, de Godoy LMF, Li G, Macek B, Mortensen P, Pesch R, Makarov A, Lange O, Horning S, Mann M: Parts per million mass accuracy on an orbitrap mass spectrometer via lock mass injection into a C-trap. Mol Cell Proteomics 2005, 4:2010-2021.