Single cell RNA-sequencing on aged mouse lung mesenchymal cells.

QC, integration, mesenchymal fraction extraction and purity confirmation were performed as described previously. The mesenchymal cell subpopulations identified in the aged normal and fibrotic lungs were similar to those identified in adult mouse lungs and each cluster showed a distinct gene expression profile. As IPF is a disease of aging, to further investigate pulmonary fibroblast lineage and the subtypes in aged and disease lungs, mesenchymal cells (EPCAM/CD31/CD45-) were collected from three control aged mouse lungs and aged matched lungs 14 days after bleomycin injury.