Microbial genomic DNA was extracted from the root, rhizosphere and bulk soil compartments from grassland phytometer plant species and was then used as templates for bacterial 16S and/or fungal ITS rDNA paired-end amplicon sequencing using Illumina MiSeq

Investigation of the root and rhizosphere microbial community composition of forb and grass phytometer plants out planted in sites of different land-use intensity in the German Biodiversity Exploratories (Hainich-Dün) by means of 16S and ITS rDNA paired-end amplicon sequencing using Illumina MiSeq. The study focused on the effect of plant functional group (grasses vs. forbs) and land-use as well as edaphic parameters onto the bacterial and fungal community and diversity.