ONT direct RNA sequencing Fast5 files from mouse embryonic stem cell (C57BL/6J x CAST/EiJ) - Rep2 (run3-2)

We present a novel approach that leverages Oxford Nanopore direct RNA sequencing technology to detect allele-biased patterns of N6-methyladenosine (m6A) modifications in native mRNAs. Our approach utilized human and mouse cells with known genetic variants to ascertain allelic origin of each mRNA molecule. We employed a supervised machine learning model to detect read-level modification ratios, providing a comprehensive understanding of allele-specific m6A modification (ASM) patterns. To analyze the effects of allele specific m6A modification in mouse, we used hybrid mosue embryonic stem cells (C57BL/6J x CAST/EiJ). This page contains replicate 2 (run 3-2; the files are splited two two parts because of limitations of file size).本研究提出一种全新方法，借助牛津纳米孔（Oxford Nanopore）直接RNA测序（direct RNA sequencing）技术，可检测天然mRNA中N6-甲基腺嘌呤(N6-methyladenosine, m6A)修饰的等位基因偏好性模式。本方法采用携带已知遗传变异的人类与小鼠细胞，以确定每条mRNA分子的等位基因来源；并通过有监督机器学习模型检测读段水平的修饰比例，从而全面解析等位基因特异性m6A修饰(allele-specific m6A modification, ASM)模式。为分析小鼠体内等位基因特异性m6A修饰的调控效应，本研究使用了杂交小鼠胚胎干细胞（C57BL/6J × CAST/EiJ）。本页面包含重复实验2（测序批次3-2；由于文件大小限制，数据文件被拆分为两部分）。