Example of Neutrophil Isolation Purity

This is an example of a purity check done on isolated neutrophils from 5-month-old female C57Bl/6J mice. Neutrophil markers Ly6G and Cd11b were used. We compared the Stemcell EasySep Neutrophil Enrichment Kit (E1 and E2) to the Miltenyi Biotec Neutrophil Isolation Kit (M1 and M2). Neutrophil samples were analyzed using the MacsQuant10 and gating was performed using FlowLogic version 8. The files begin with 2021-08-24.Additionally, we performed a purity estimate on 5 young (4-months-old) female and 5 young male C57BL/6J mice. Neutrophil markers Ly6G and Cd11b were used. Neutrophil samples were analyzed using the Macsquant10 and gating was performed using FlowLogic version 8. The files begin with 2019-07-30.本数据集为针对5月龄雌性C57Bl/6J小鼠分离得到的中性粒细胞所开展的纯度检测示例。实验采用中性粒细胞标志物Ly6G与Cd11b进行标记。本研究对比了Stemcell公司EasySep中性粒细胞富集试剂盒（编号E1、E2）与Miltenyi Biotec公司中性粒细胞分离试剂盒（编号M1、M2）的分选效果。中性粒细胞样本通过MacsQuant10流式细胞仪完成分析，门控分析采用FlowLogic 8.0版本软件进行，相关数据文件的命名前缀均为2021-08-24。此外，本研究还对5只4月龄雌性、5只4月龄雄性C57BL/6J小鼠开展了中性粒细胞纯度评估实验，同样采用Ly6G与Cd11b作为中性粒细胞标志物。样本分析通过MacsQuant10流式细胞仪完成，门控分析使用FlowLogic 8.0版本软件进行，该批次数据文件的命名前缀均为2019-07-30。