Gliding motility assay using 11040mM D2O in solution. Replicate 1/3. ROI 9/15.

• This is a gliding motility assay using kinesin-1 and rhodamine labeled microtubules. • The buffer used was PEM (also known as BRB80) with the inclusion of 11040mM D2O. • Images were taken with an Andor Luca EMCCD camera on an Olympus IX-71 microscope using a 60x 1.42NA PlanApo objective. • This fileset is replicate 1/3 and ROI 9/15. • Images were taken to investigate speed variations of microtubules due to the inclusion of D2O in the motility assay. • The temperature of the slide was held constant at 32°C by an objective heater. Early ROIs, i.e. 1-5, have slower giding speeds due to the increasing temperature of the slide which, was prepared at room temperature before observation. After ROI 5 the gliding speed stabilizes. • Pixels lengths were measured to be 166nm/pixel using a stage micrometer. • After the files have downloaded, a simple command line entry will convert the images to a movie. Mencoder must be installed. ls > list.txt | mencoder mf://@list.txt -mf w=496:h=658:fps=30:type=png -ovc lavc -lavcopts vcodec=mpeg4:mbd=2:trell -o output.avi • Upload of these files were made possible through the figshare API.• 本数据集为采用驱动蛋白-1（kinesin-1）与罗丹明标记微管的滑行运动测定实验。 • 实验所用缓冲液为PEM（又称BRB80），并添加了11040mM 重水（D₂O）。 • 图像采集采用Olympus IX-71型显微镜，搭配Andor Luca电子倍增CCD（EMCCD）相机与60× 1.42NA PlanApo物镜完成拍摄。 • 本次文件集对应第1/3组重复实验与第9/15个感兴趣区域（ROI）。 • 本实验旨在探究运动测定体系中添加重水（D₂O）对微管滑行速度的影响。 • 通过物镜加热器将载玻片温度恒定维持在32°C。早期感兴趣区域（即1-5号ROI）因载玻片观测前以室温制备，观测过程中温度逐渐升高，滑行速度较慢；5号ROI之后，微管滑行速度趋于稳定。 • 使用载物台测微尺校准得到每像素对应长度为166nm。 • 文件下载完成后，可通过以下简单命令行指令将图像批量转换为视频，需预先安装Mencoder软件：ls > list.txt | mencoder mf://@list.txt -mf w=496:h=658:fps=30:type=png -ovc lavc -lavcopts vcodec=mpeg4:mbd=2:trell -o output.avi • 本数据集文件通过figshare API接口完成上传。