Genome-wide analysis of total skin gene expression from presenilin (1&2)-null (PSmut), RBPjk-null (RBPjmut), Presenilin (1&2)-RBPjk-null (PSRmut) and wild-type (WT) littermates. Mus musculus

Analysis of differential gene expression of total skin from different Notch signaling pathway mutants (specifically PSmut, RBPjmut, and PSRmut). Preselin1&2 are the core components of g-secretase enzyme which essential for the Notch receptor activation. RBPjk is the DNA-binding partner of the Notch Intracellular Domain. The hypothesis tested in the present study was RBPjmut skin would resemble the wild-type skin at the transcriptional level, since RBPjmut mice exhibit milder phenotype than PSmut or PSRmut mice. Overall design: Total RNA obtained from 4x RBPjmut, 4x PSmut, 4x PSRmut total skin at P0 and compared to wild-type total skin from littermates. All mice were raised in mixed genetic background.